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1989) in a volume of 20 µl with 1.0 unit of Ampli Taq Gold (Applied Biosystems Inc., Foster City, California), 1× polymerase chain reaction buffer, 0.2 m M of each deoxynucleoside triphosphate, 0.5 µM of each primer, 2 m M of Mg Cl, and 1 µ1 of template DNA.
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