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A total of 28 young (4 m) and aged (24 m) male rats were divided into four equal groups: group 1 – young control; group 2 – young trained; group 3 – old control and group 4 – old trained group.

At the end of 8 weeks, rats were sacrificed and penile tissues evaluated for e NOS and n NOS activities.

e NOS and n NOS activities were evaluated by immunohistochemistry in paraffinized penile tissues and results assessed semiquantitatively.

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We can suggest that regular exercise upregulates e NOS and n NOS expressions in the aged and young rat penis.

Regular exercise may improve penile erection by increasing penile neurotransmitter in both young and aged rats.

In penile tissues of aged control rats, e NOS and n NOS staining were weakly positive; however in trained groups, e NOS and n NOS immunoreactivity were increased.

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